human nb cell line sh sy5y  (ATCC)

Journal: iScience

Article Title: Real-time morphometric analysis of targeted therapy for neuroblastoma cells in monolayer and 3D hydrogels using digital holographic microscopy

doi: 10.1016/j.isci.2024.111231

Figure Lengend Snippet: Growth rate morphometric parameters and movement pattern of SK-N-BE (2) and SH-SY5Y cells in monolayer cultures with and without CLG (A) Holographic micrographs of a representative focus position obtained at 0 h and 20 h with and without CLG treatment of SK-N-BE (2) (right) and SH-SY5Y (left) cells. The small inset graph represents the optical thickness of the cell aggregate measured along the red and green lines in control and CLG treatment groups, respectively. The dotted line shows the maximum thickness in each graph. The number of cells is N = 97 (0 h) and N = 150 (24 h) in SK-N-BE (2) and N = 75 (0 h) and N = 118 (24 h) in SH-SY5Y. (B) Measurement over time of optical volume (μm 3 ), optical thickness (μm), and irregularity (0 = smooth, 1 = rough) of cell micrographs shown in (A). (C) Movement of the highlighted cell with long-distance trajectory from each micrograph in control and CLG treatment groups. Rose plots depict relative single-cell tracking X-Y migration showing the similarities (more symmetry) and differences (less symmetry) in movement from all cells. Arrows highlight the movement of the depicted cell. The field of view is 567 × 567 μm. The scale bar is 100 μm. Brighter color represents maximum cell height. CLG, Cilengitide.

Article Snippet: MYCN -amplified SK-N-BE (2) and ALK -mutated SH-SY5Y human NB cell lines were chosen from a variety of available cell lines, since MYCN -amplified and ALK -mutated tumors represent 64% of high-risk neuroblastoma [50 and 14%, respectively]., Cells were acquired from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in IMDM medium (Gibco, Life Technologies, Waltham, MA, USA) supplemented with 10% FBS, 1% insulin/transferrin and 1% Penicillin/streptomycin at 37°C in 5% CO2 atmosphere.

Techniques: Control, Single Cell Tracking, Migration

Journal: iScience

Article Title: Real-time morphometric analysis of targeted therapy for neuroblastoma cells in monolayer and 3D hydrogels using digital holographic microscopy

doi: 10.1016/j.isci.2024.111231

Figure Lengend Snippet: Long-term movement pattern in hydrogel-free 2D-grown SK-N-BE (2) and SH-SY5Y cell lines at the hydrogel border with and without CLG (A) Representative holographic micrographs from hydrogel-grown SK-N-BE (2) (right) and SH-SY5Y (left) cell lines with selected hydrogel-free-grown cells at the non-VN-added and VN-rich hydrogel border at day 10 of culture (time point: 24 h). Arrows and arrow heads highlight cells growing in monolayer and cell aggregates, respectively. The border is highlighted with a dotted line. (B) Rose plots depict relative single-cell tracking X-Y migration showing the similarities (more symmetry) and differences (less symmetry) in cell movement of all selected cells from up to three different focus positions from each condition. The field of view is 567 × 567 μm. The scale bar is 100 μm. CLG, Cilengitide; VN, vitronectin.

Article Snippet: MYCN -amplified SK-N-BE (2) and ALK -mutated SH-SY5Y human NB cell lines were chosen from a variety of available cell lines, since MYCN -amplified and ALK -mutated tumors represent 64% of high-risk neuroblastoma [50 and 14%, respectively]., Cells were acquired from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in IMDM medium (Gibco, Life Technologies, Waltham, MA, USA) supplemented with 10% FBS, 1% insulin/transferrin and 1% Penicillin/streptomycin at 37°C in 5% CO2 atmosphere.

Techniques: Single Cell Tracking, Migration

Journal: iScience

Article Title: Real-time morphometric analysis of targeted therapy for neuroblastoma cells in monolayer and 3D hydrogels using digital holographic microscopy

doi: 10.1016/j.isci.2024.111231

Figure Lengend Snippet: Comparison of cluster morphometric parameters of SK-N-BE (2) and SH-SY5Y cells over 3, 7, and 10 days of culture in non-VN-added and VN-rich 3D GTA-sf hydrogels with and without CLG (A) Representative holographic micrographs from VN-rich hydrogel-grown SK-N-BE (2) and SH-SY5Y cell clusters without CLG at day 3, 7, and 10 of culture. Red, green, and blue arrows depict the 24 h-length videos taken at 0 and 24 h. (B–D) Morphometric parameters in SK-N-BE (2) and SH-SY5Y cell clusters at 0 h and 24 h of day 3 (red line), 7 (green line), and 10 (blue line) time points in non-VN-added and VN-rich hydrogels without CLG (upper graphs) and with CLG (lower graphs). (B) Optical volume (μm)3, (C) optical thickness (μm), and (D) irregularity (0–1) measurements. Data are represented as mean ± SEM. (E) Heatmap of statistically significant differences of SK-N-BE (2) and SH-SY5Y cell clusters at 3, 7, and 10 day time points between non-VN-added and VN-rich hydrogels without CLG and with CLG. (i) Optical volume (μm)3, (ii) optical thickness (μm), and (iii) irregularity (0–1) measurements. SK: SK-N-BE (2), SH: SH-SY5Y, nVN-C: non-VN-added Control, nVN-CLG: non-VN-added CLG, VN-C: VN-rich-Control, VN-CLG: VN-rich-CLG. Statistical analysis using non-parametrical Kruskal-Wallis test, ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001. SEM, standard error of the mean. Significant differences discussed in the text are highlighted in bold. The field of view is 567 × 567 μm. The scale bar is 100 μm. D, day, CLG, Cilengitide, VN, vitronectin.

Article Snippet: MYCN -amplified SK-N-BE (2) and ALK -mutated SH-SY5Y human NB cell lines were chosen from a variety of available cell lines, since MYCN -amplified and ALK -mutated tumors represent 64% of high-risk neuroblastoma [50 and 14%, respectively]., Cells were acquired from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in IMDM medium (Gibco, Life Technologies, Waltham, MA, USA) supplemented with 10% FBS, 1% insulin/transferrin and 1% Penicillin/streptomycin at 37°C in 5% CO2 atmosphere.

Techniques: Comparison, Control

Journal: iScience

Article Title: Real-time morphometric analysis of targeted therapy for neuroblastoma cells in monolayer and 3D hydrogels using digital holographic microscopy

doi: 10.1016/j.isci.2024.111231

Figure Lengend Snippet:

Article Snippet: MYCN -amplified SK-N-BE (2) and ALK -mutated SH-SY5Y human NB cell lines were chosen from a variety of available cell lines, since MYCN -amplified and ALK -mutated tumors represent 64% of high-risk neuroblastoma [50 and 14%, respectively]., Cells were acquired from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in IMDM medium (Gibco, Life Technologies, Waltham, MA, USA) supplemented with 10% FBS, 1% insulin/transferrin and 1% Penicillin/streptomycin at 37°C in 5% CO2 atmosphere.

Techniques: Recombinant, Software, Imaging